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. 2000 Jan 4;97(1):343–348. doi: 10.1073/pnas.97.1.343

Figure 3.

Figure 3

Radioimmunoprecipitation analysis of the wt (NL4–3) and the CTdel-144–2 mutant. HeLa (A), MT-4 (B), CEM (12D-7) (C), or Jurkat (D) cells were infected with VSV-G-pseudotyped KFS (Env-minus), NL4–3, or CTdel-144–2 and metabolically labeled overnight with [35S]Cys. Virion-associated material was obtained by pelleting the infected cell supernatant in an ultracentrifuge; lysates derived from cell- and virion-associated material were immunoprecipitated with AIDS patient sera (see Materials and Methods). In Jurkat cells (D), virus-free supernatant obtained from the ultracentrifuge spin also was immunoprecipitated. (E) Radioimmunoprecipitation analysis of MDM infected with the VSV-G-pseudotyped, macrophage-tropic molecular clone NL(AD8) expressing wt or CTdel-144–2-mutant Env. Radioimmunoprecipitation was performed as described above except metabolic labeling was performed with [35S]Met. The positions of the Env precursor gp160, the mature surface glycoprotein gp120, p66(RT), the Gag precursor Pr55Gag, p32(IN), and p24(CA) are indicated. The results are representative of at least two independent experiments.