Figure 4.

Relative MAGI infectivity of virions derived from MT-4 (Left) or CEM (12D-7) (Right) cells. Virus preparations were obtained by cotransfecting HeLa cells with HIV-1 molecular clones (pNL4–3, pNL4–3CTdel-144–2, or pNL4–3KFS) and a VSV-G expression vector. Stocks were normalized for RT activity and used to infect MT-4 and CEM (12D-7) cells. Two days postinfection, the infectivity of NL4–3, NL4–3CTdel-144–2, or NL4–3KFS virus recovered from the T cell line supernatant was determined by MAGI assay (Materials and Methods). Data presented are averages of at least two assays, ±SD.