TABLE 2.
Meiotic effects in Sxl mutant females
| Effect on meiosis
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Recombination
|
% nondis-junction of X | |||||||
| Cross | Relevant female genotype | Infected? | Genetic interval (chromosome) | Progeny scoreda | Observed distance (cM) | % book value | Magnitude of effect (cross/cross) | |
| 1. Absence of a Wolbachia effect | ||||||||
| A1 | SxlM6,f3/Sxlf4 | No | y-sn (X) | 4563 | 2.4 | 12 | A1/B = 16% | 1.6 |
| A2 | SxlM6,f3/Sxlf4 | Yes | y-sn (X) | 4697 | 2.3 | 11 | A2/B = 15% | 1.0 |
| B | SxlM6,f3/Sxlf4; Dp(Sxl+)/+ | No | y-sn (X) | 3953 | 14.9 | 71 | (Sxl+ control) | <0.1 |
| 2. Dominant effect of SxlM6,f3 | ||||||||
| C1 | SxlM6,f3/+ | No | y-ct (X) | 1574 | 13.5 | 68 | C1/C2 = 68% | 0.1 |
| C2 | SxlM6,f3/+; Dp(Sxl+)/+ | No | y-ct (X) | 877 | 19.8 | 99 | (Sxl+ control) | 0.1 |
| 3. Dominant synergism between Sxl and otu− | ||||||||
| D1 | SxlM1,f3/Sxlf18 | No | y-ct (X) | 3319 | 3.9 | 20 | (otu+ control) | 0.5 |
| D2 | SxlM1,f3 otu−/ Sxlf18 + | No | y-ct (X) | 2989 | 1.3 | 7 | D2/D1 = 33% | 4.2 |
| E1 | SxlM1,f3 otu−/+ + | No | y-ct (X) | 4957 | 6.5 | 33 | E1/E2 = 48% | 1.7 |
| E2 | SxlM1,f3/+ | No | y-ct (X) | 4358 | 13.6 | 68 | (otu+ control) | <0.1 |
| F | Sxlf18/+ | No | y-ct (X) | 3930 | 19.4 | 97 | <0.1 | |
| G1 | otu−/+ | No | y-ct (X) | 8497 | 11.8 | 59 | G1/G2 = 99% | 0.9 |
| G2 | +/+ | No | y-ct (X) | 6647 | 11.9 | 60 | (otu+ control) | 0.4 |
| 4. Differential sensitivity of X vs. autosome in Wolbachia-suppressed Sxlf4 | ||||||||
| H | Sxlf4/Sxlf4 | Yes | w-sn (X) | 413 | 0 | <1 | 30 | |
| sn-f (X) | 99 | 0 | <3 | |||||
| al-b-c-sp (II) | 218 | 11 | 10 | H/J2 = 11% | ||||
| 5. Sxlf24,M1 complements Sxlf4 well for fertility but poorly for meiosis | ||||||||
| J1 | Sxlf24,M1/Sxlf4 | No | y-cv-f (X) | 2437 | 0.2 | 0.4 | J1/J2 = 0.5% | 24 |
| al-dp-b-pr-cn-c-px-sp (II) | 3054 | 5.0 | 5 | J1/J2 = 5% | ||||
| J2 | Sxlf24,M1/Sxlf4; Dp(Sxl+)/+ | No | y-cv-f (X) | 983 | 38.5 | 68 | (Sxl+ control) | ND |
| al-dp-b-pr-cn-c-px-sp (II) | 1540 | 102.9 | 96 | (Sxl+ control) | ||||
| K1 | Sxlf24,M1/Sxlf4 | No | y-cv-f (X) | 693 | 0 | <0.2 | K1/K2 = <0.4% | ND |
| ru-h-th-st-cu-sr-e (III) | 2584 | 4.4 | 6 | K1/K2 = 7% | ||||
| K2 | Sxlf24,M1/Sxlf4; Dp(Sxl+)/+ | No | y-cv-f (X) | 1532 | 35.5 | 63 | (Sxl+ control) | ND |
| ru-h-th-st-cu-sr-e (III) | 987 | 62.5 | 88 | (Sxl+ control) | ND | |||
Full genotype of crosses:
A1 and A2: y w cv SxlM6,f3 ct6/w cm Sxlf4 sn3 (U) and w cm Sxlf4 sn3/y w cv SxlM6,f3 ct6 (I) ♀̆, respectively, × ♂♂ y cm ct6 sn3 v/Y. Infected and uninfected mothers were from reciprocal crosses between an infected Sxlf4 stock and uninfected SxlM6,f3 stock. Twelve mothers for each cross.
B: y w cv SxlM6,f3 ct6/w cm Sxlf4 sn; Dp(1;3)sn13a1, cm+ Sxl+ct+/+ ♀̆ × ♂♂ y cm ct6 sn3 v/Y. Data for 10 mothers.
C1 and C2: y w cv SxlM6,f3 ct6/w sisters without (12♀̆) and with Dp(1;3)sn13a1,Sxl+ (10♀̆), respectively, ♀̆ × ♂♂ y cm ct6 sn3 v /Y. Recombination value is based on daughters only.
D1: y w SxlM1,f3 ct6 sn3/w Sxlf18 g f ♀̆ and ♀̆♀̆ × ♂♂ y v sisA m g f/Y. Data for 17 mothers. Recombination value is based on sons only.
D2: y w SxlM1,f3 ct6 otu17/w Sxlf18 g f ♀̆ and ♀̆♀̆ × ♂♂ y v sisA m g f/Y. The y-ct region on the SxlM1,f3 chromosome is identical to that of the SxlM1,f3 chromosome in Cross D1. Data for 25 mothers. Recombination value is based on sons only.
E1: y w SxlM1,f3 ct6 otu17/w ♀̆ × ♂♂ y cm ct6 sn3 v/Y. Data based on 10 mothers.
E2: y w SxlM1,f3 ct6 sn3/w ♀̆ × ♂♂ y cm ct6 sn3 v/Y. The y-ct region on the SxlM1,f3 chromosome is identical to that of the SxlM1,f3 chromosome in Cross E1. Data based on 10 mothers.
F: w Sxlf18 g f/y cm ct6 ♀̆ × ♂♂ y cm ct6 sn3 v/Y. Data for 6 mothers.
G1: y cm ct6 otu17/w ♀̆ × ♂♂ y cm ct6 sn3 v/Y. Nondisjunction is based on XO only. Data for 17 mothers.
G2: y cm ct6 sn3/w ♀̆ × ♂♂ y cm ct6 sn3 v/Y. The y-ct region on the w+ chromosome is identical to that of the w+ chromosome in cross G1. Nondisjunction is based on XO only. Data for 10 females.
H: w cm Sxlf4 sn/cm Sxlf4 v f; al b c sp/+ ♀̆♀̆ × ♂♂ y w cm ct6 sn3/Y; al b c sp/CyO. sn-f distance based on w+sn+ sons only. Data for first 9 days of progeny from 300 mothers (50/vial).
J1 and J2: y cv cm Sxlf24,M1/cm Sxlf4 v f; al dp b pr cn c px sp/+ sisters without and with Dp(1;3)sn13a1,Sxl+, respectively, ♀̆♀̆ × ♂♂ +/Y; al dp b pr cn c px sp/CyO. Data for progeny of 230 mothers and 50 mothers, respectively, collected for 6 days (50/vial).
K1 and K2: y cv cm Sxlf24,M1/cm Sxlf4 v f; ru h th st cu sr e ca /+ sisters without and with Dp(1;3)sn13a1,Sxl+, respectively, ♀̆♀̆ × ♂♂ +/Y; ru h th st cu sr e ca. Data for 200 and 50 mothers, respectively, (50/vial). K1 progeny were collected for 9 days, while K2 progeny were collected over the same period, but progeny from days 3, 6, and 7 were not scored.
Progeny scored are those on which the recombination rate calculations were made. Unless otherwise stated, progeny were from the first 10 days of laying.