Figure 6.—

Global changes in gene expression occur in histone methyltransferase mutant Drosophila. (A) Venn diagram illustrating overlap of misregulated genes in Su(var)3-9⁰⁶ (red) and egg²³⁵/+ (blue) single mutants as well as the egg²³⁵/+; Su(var)3-9⁰⁶ (yellow) double mutant. At the bottom of each genotype, the total number of misregulated genes detected is given. RNA prepared from fly heads. (B) Bar chart depicting the percentage of misregulated genes in each mutant background compared to wild type by chromosome. A high proportion of fourth chromosome genes are misregulated as a consequence of the dSETDB1 mutation. (C) Bar chart depicting the percentage of misregulated genes in each heterochromatin domain. (D) Bar chart illustrating transposon reactivation in each mutant background based on increased expression levels compared to wild type. Loss of SU(VAR)3-9 activity has the more pronounced effect on transposon expression; this is frequently reversed in the double-mutant line.