TABLE 2.
Differential response of individual transcripts
| Target gene (transcript)a | Genotypeb
|
Significant genotypesc | Total no. of transcripts | Alternative promoters? | |||
|---|---|---|---|---|---|---|---|
| Htlact | Egfract | Ras1act | Pntact | ||||
| CG9641 | 0 | 0 | 0 | 0 | None | 2 | Uncertain |
| (CG9641-RA) | 0 | −− | 0 | +++ | Egfract, Pntact | ||
| cher | NA | NA | NA | NA | NA | 4 | Yes |
| (cher-RA/RD) | 0 | −−− | ++++ | ++++ | Htlact, Egfract | ||
| (cher-RB) | +++ | +++ | +++ | +++++ | Pntact | ||
| (cher-RC) | +++ | + | +++++ | +++ | Egfract, Ras1act | ||
| (cher-RD) | ++ | 0 | +++ | +++++ | Egfract, Pntact | ||
| l(2)08717 | 0 | −/−− | 0 | ++ | Egfract, Pntact | 2 | Yes |
| (l(2)08717-RB) | ++ | −− | +/++ | −−/−−− | Noned | ||
| pax | NA | NA | NA | NA | NA | 6 | Yes |
| (pax-RA) | +++ | ++ | +++ | +++ | Egfract | ||
| (pax-RB) | +++++ | ++ | +++/++++ | ++ | Htlact, Ras1act | ||
| (pax-RC) | ++++ | 0 | +++ | +++ | Egfract, Htlact | ||
| (pax-RD) | 0 | − | 0 | +++ | Egfract, Pntact | ||
| smi35A | ++/+++ | 0 | ++ | −−/−−− | Egfract, Pntact | 5 | No |
| (smi35A-RC/RD) | +++ | 0 | ++ | −−−− | All different | ||
“Target gene” refers to the common portion for all transcripts of the gene; “transcript” refers to results for the indicated transcript only.
Results are from from quantitative real-time PCR with RNA from the indicated genotype. + indicates a positive change in expression relative to wild type, − a negative change, and 0 no significant change. Change is represented by + or − (for increased and decreased expression, respectively); magnitude is given in log2 fold-change ranges as follows: log2 fold change = 0–0.5, +; 0.5–1.0, ++; 1–1.5, +++; 1.5–2.0, ++++; >2.0, +++++. NA, qRT–PCR not performed for a portion of mRNA common to all transcripts.
“Significant genotypes” indicates genotypes for which the target gene expression is significantly different from all other genotypes (adjusted P < 0.05).
Egfract and Pntact are similar; Htlact and Ras1act are similar.