TABLE 1.
Male gametophytic mutants grouped by phenotypic category
SGT name | Line name | Annotation and/or female gametophytic defect | Insertion sitea and Ds orientationb | TE (KanR/KanS)
|
|||
---|---|---|---|---|---|---|---|
AGI ID | Self | Male | Female | ||||
Pollen development defective (PDD) | |||||||
SGT 08397 | pdd1 | At3g10490c | Arabidopsis NAC domain protein, ANAC051, ANAC052 | 141 bp upstream | 1.1 | 0.0 | 0.9 |
SGT 07406 | pdd2 | At2g01820c | LRR kinase | 12 bp upstream | 0.7 | 0.0 | 0.8 |
SGT 09930 | pdd3 | At5g63890 | Histidinol dehydrogenase (At HDH) | 145 bp upstream; 5′–3′ | 0.7 | 0.0 | 0.6 |
SGT 14109 | pdd4 | At4g13890c | Serine hydroxymethyltransferase 5 (SHM5), EDA37 | Exon 2 | 0.2 | 0.0 | 0.4 |
SGT 01712 | pdd5 | At2g34880c | Transcription factor jumonji (jmj), MEE27 | 381 bp upstream | 0.2 | 0.0 | 0.3 |
SGT 03118 | pdd6 | At2g35210 | ARF-GAP-like (At AGD10), MEE28 | Exon 3; 5′–3′ | 0.1 | 0.0 | 0.3 |
SGT 05238 | pdd7 | At2g34830/35c | Intergenic, 2.6 kb upstream of At2g34835 and 2.5 kb upstream of At2g34830 | 0.2 | 0.0 | 0.3 | |
SGT 01706 | pdd8 | Unannotated | Upstream small ORF, 53 aa (ath_mu_ch2_12997 bottom) | Intergenic, 1.2 kb upstream of At2g34850 and 1.3 kb upstream of At2g34860; 5′–3′ | 0.2 | 0.0 | 0.3 |
SGT 02837 | pdd9 | At2g34920c | Protein binding/zinc ion binding, EDA18 | Exon 4 | 0.3 | 0.0 | 0.3 |
SGT 04915 | pdd10 | At2g37690c | Phosphoribosylaminoimidazole carboxylase | 33 bp upstream | 0.1 | 0.0 | 0.3 |
SGT 04025 | pdd11 | At2g35060/70c | Intergenic, 2.8-kb At2g35060 and 2.5 kb upstream of At2g35070 | 0.6 | 0.0 | 0.4 | |
SGT 02824 | pdd12 | At2g34220 | Unknown protein, MEE20 | Exon 3; 5′–3′ | 0.2 | 0.0 | 0.3 |
SGT 00458 | pdd13 | At4g04040–At4g04870 | MEE51 | 525 kb deletion; 5′–3′ | 0.3 | 0.0 | 0.1 |
SGT 01526 | pdd14 | At3g10000/At2g34850/60 | Translocation | 0.2 | 0.0 | 0.2 | |
SGT 14667 | pdd15 | At5g44700–At5g44760 | EDA23 | 26-kb deletion; 5′–3′ | 0.2 | 0.0 | 0.1 |
SGT 08841 | pdd16 | At2g01200c | AUX/IAA family protein, IAA32, MEE10 | Exon 1 | 0.6 | 0.1 | 0.9 |
SGT 03904 | pdd17 | At4g14790 | RNA helicase (At SUV3) | 108 bp upstream; 3′–5′ | 0.5 | 0.1 | 0.4 |
SGT 09368 | pdd18 | At1g60870c | Expressed protein, MEE 9 | 44 bp downstream | 0.5 | 0.4 | 0.4 |
SGT 06608 | pdd19 | At1g79860–At1g80740 | MEE64 | 300-kb deletion; 5′–3′ | 0.5 | 0.4 | 0.5 |
SGT 06991 | pdd20 | At5g58230 | MSI1, MEE70 | Exon 1; 5′–3′ | 0.9 | 0.4 | 0.0 |
SGT 13017 | pdd21 | At3g10405c | Unknown protein | 190 bp upstream | 0.5 | 0.0 | 0.5 |
SGT 08584 | pdd22 | At4g00700–At4g00910 | 100-kb deletion; 3′–5′ | 0.6 | 0.0 | 0.8 | |
SGT 06569 | pdd23 | At3g56200c | Polyamine transporter | Exon 1 | 1.5 | 0.0 | 1.1 |
SGT 02152 | pdd24 | At3g10960/70c | Intergenic, 2.2 kb downstream of At3g10970 and 3.9 kb upstream of At3g10960 | 0.6 | 0.0 | 0.5 | |
SGT 02381 | pdd25 | At4g00330 | Calmodulin-binding receptor-like kinase (CRCK2) | Exon 1; 5′–3′ | 1.0 | 0.0 | 0.8 |
SGT 03906 | pdd26 | At4g14790 | RNA helicase (AtSUV3) | 130 bp upstream; 3′–5′ | 0.6 | 0.0 | 0.7 |
SGT 13701 | pdd27 | At4g01530/At2g47470 | Translocation | 1.0 | 0.1 | 1.0 | |
SGT 03135 | pdd28 | At2g34850/60c | Intergenic, 1.1 kb upstream of At2g34860 and 1.2 kb upstream of At2g34850 | 1.0 | 0.2 | 0.7 | |
SGT 11685 | pdd29 | At4g36170/At2g01420 | Translocation | 1.0 | 0.3 | 1.0 | |
SGT 04228 | pdd30 | Unannotated | Upstream small ORF, 106 aa; GenBank EST EG515492 | Intergenic, 2 kb upstream of At1g26810 and 1.3 kb downstream of At1g26820; 3′–5′ | 1.5 | 0.3 | 0.8 |
SGT 04087 | pdd31 | At2g34825/At2g34830 | AtWRKY35 | 476 bp downstream of At2g34830 and 1.8 kb downstream of At2g34825; 3′–5′ | 0.2 | 0.0 | 0.4 |
SGT 12162 | pdd32 | At3g63290c | Unknown protein | Exon 1 | 1.0 | 0.2 | 0.3 |
SGT 14110 | pdd33 | At4g13890/At2g01200 | EDA36 | Translocation | 0.4 | 0.0 | 0.6 |
SGT 09926 | pdd34 | At5g54740c | Lipid transfer protein (LTP) | 330 bp upstream | 1.3 | 0.3 | 1.0 |
Pollen germination defective (PGD) | |||||||
SGT 08289 | pgd2 | At3g10620–At3g11270 | 200-kb deletion; 5′–3′ | 0.9 | 0.0 | 1.0 | |
SGT 07358 | pgd3 | At3g10560c | Cytochrome P450 (CYP77A7),UNE9 | Exon | 0.4 | 0.0 | 0.6 |
SGT 03671 | pgd4 | At2g35070 | Unknown protein | Intron 1; 5′–3′ | 0.1 | 0.0 | 0.3 |
SGT 03669 | pgd5 | At2g34090c | Unknown protein, MEE18 | 71 bp upstream | 0.2 | 0.0 | 0.3 |
SGT 01084 | pgd6 | At4g05450 | Adrenodoxin-like ferredoxin 2 | 315 bp upstream; 5′–3′ | 0.3 | 0.1 | 0.1 |
SGT 03063 | pgd7 | At2g35300 | LEA group 1 domain protein | 70 bp downstream; 3′–5′ | 1.2 | 0.0 | 0.9 |
SGT 01001 | pgd8 | At3g05770 | Unknown protein | Intron 1; 5′–3′ | 0.7 | 0.1 | 1.0 |
SGT 11239 | pgd9 | At3g10405–At3g10116 | 110-kb deletion; 3′–5′ | 0.7 | 0.0 | 0.7 | |
Pollen tube growth defective (TGD) | |||||||
SGT 01850 | tgd1 | At3g03810c | Unknown protein, EDA30 | 141 bp downstream | 0.3 | 0.0 | 0.2 |
SGT 04586 | tgd2 | At2g34850c | Intergenic, 426 bp downstream of At2g34850 | 0.7 | 0.4 | 0.4 | |
SGT 07405 | tgd3 | At3g07330c | Cellulose synthase-like C6 (ATCSLC6) | Exon 1 | 1.5 | 0.5 | 0.7 |
SGT 03295 | tgd4 | At2g12945/At5G54745 | Translocation | 1.1 | 0.6 | 0.4 | |
SGT 05752 | tgd5 | At1g77131 | Pseudogene of PGSIP, glycogenin glycosyltransferase | 754 bp upstream of At1g77130; 3′–5′ | 1.0 | 0.0 | 1.0 |
SGT 13609 | tgd6 | Unannotated | Downstream small ORF, 53 aa | Intergenic, 1.8 kb downstream of At4g11550 and 2.3 kb upstream of At4g11540; 3′–5′ | 1.2 | 0.4 | 0.9 |
SGT 11205 | tgd7 | At3g06530/At3g06540 | Intergenic, 771 bp downstream of At3g06540 and 750 bp downstream; 3′–5′ | 1.3 | 0.1 | 1.0 | |
SGT 02510 | tgd8 | At3g05000 | Transport protein particle (TRAPP) component, Bet3 family protein | Exon 1; 5′–3′ | 1.0 | 0.3 | 1.0 |
SGT 09403 | tgd9 | At3g59760c | O-acetylserine (thiol) lyase isoform C (OASC) | Exon 1 | 1.2 | 0.6 | 0.9 |
SGT 11437 | tgd10 | At3g06430 | Pentatricopeptide repeat protein, EMB2750 | Exon 1; 5′–3′ | 1.1 | 0.6 | 0.4 |
SGT 01267 | tgd11 | At3g03650c | Exostosin family protein, EDA5 | Exon 1 | 0.2 | 0.0 | 0.6 |
SGT 00656 | tgd12 | At3g03690c | Glycosyl transferase family 14, UNE7 | Intron 1 | 0.2 | 0.0 | 0.2 |
SGT 15318 | tgd13 | At3g10280–At3g10915 | 235-kb deletion; 3′–5′ | 0.3 | 0.0 | 0.2 | |
SGT 08149 | tgd14 | At1g25240/At1g25250 | Intergenic, 1.5 kb upstream of At1g25250 and 1.2 kb downstream; 5′–3′ | 1.2 | 0.2 | 1.0 | |
SGT 04229 | tgd15 | At2g34590c | Transketolase family protein; similar to Pyruvate dehydrogenase E1 beta (PDH-E1 BETA) | 66 bp upstream | 1.4 | 0.3 | 0.8 |
SGT 15447 | tgd16 | At2g47510 | Fumarase (FUM1) | Exon 1; 3′–5′ | 1.0 | 0.5 | 0.8 |
SGT 07020 | tgd17 | At1g25450c | Very-long-chain fatty acid condensing enzyme (CUT1) | Exon 1 | 0.6 | 0.2 | 0.2 |
SGT 07170 | tgd18 | At2g47470 | UNE5 | 400 bp upstream of At2g47470; 5′–3′ | 1.2 | 0.4 | 0.6 |
SGT 14828 | tgd19 | At3g05200–At3g05320 | 30-kb deletion; 5′–3′ | 1.0 | 0.0 | 0.9 | |
Pollen tube–embryo sac interaction (PEI) and fertilization defective | |||||||
SGT 05178 | pei1 | At3g58350/At2g34850 | Translocation | 0.7 | 0.1 | 0.6 | |
SGT 12231 | pei2 | At3g14940/At3g14950 | Intergenic, 366 bp downstream of At3g14940 and 346 bp downstream of At3g14950; 5′–3′ | 1.3 | 0.4 | 0.9 | |
No obvious phenotype | |||||||
SGT 11748 | At1g04620/30/ At2g34850/60 | Translocation | 0.3 | 0.4 | 0.1 | ||
SGT 12347 | At2g15890c | Unknown protein, MEE14 | Exon 1 | 0.5 | 0.4 | 0.1 | |
SGT 01214 | At3g14920–At3g15030 | MEE35 | 44-kb deletion; 5′–3′ | 0.5 | 0.4 | 0.1 |
The genetrap (SGT) number is indicated. The annotation is according to TAIR. Transmission efficiency (TE) (KanR/KanS) is shown from self and reciprocal crosses. The female gametophyte phenotype noted by Pagnussat et al. (2005) is indicated, if present. AGI ID, Arabidopsis Genome Initiative identification number.
The insertion location relative to the transcribed region of the annotated gene.
Orientation of the Ds insertion relative to the transcribed region of the annotated gene (shown only for insertions where both flanking sequences were recovered and map to the same chromosome). For lines in which the distance from the closest ORF is >1 kb upstream or 0.3 kb downstream, the genomic coordinate of the Ds insertion is indicated.
Lines for which only one genomic flanking sequence was recovered.