TABLE 2.
Tests for complementation of Df(1)Hmr− female viability and fertility defects
| Genotype (no. of females at start) | Viability relative to Na (mean no. of progeny per female ± SD)b
|
|||||
|---|---|---|---|---|---|---|
| Cross | Day 5 | Day 10 | Day 15 | Day 20 | Day 25 | |
| 1 | Df(1)Hmr−/Df(1)Hmr− | 0.74 | 0.32 | 0.08 | 0.03 | 0.01 |
| (N = 73) | (6.2 ± 11.9) | (1.22 ± 3.9) | (0) | (0) | (0) | |
| Df(1)Hmr−/Df(1)Hmr−; P{Hmr+tp72}11-1/+ | 0.70 | 0.42 | 0.31*** | 0.22*** | 0.14** | |
| (N = 73) | (54.5 ± 33.3)*** | (43.9 ± 23.8)*** | (52.9 ± 19.1)*** | (49.6 ± 22.4)** | (55.8 ± 35.4) | |
| 2 | Df(1)Hmr−/Df(1)Hmr− | 0.77 | 0.40 | 0.22 | 0.05 | 0.02 |
| (N = 65) | (14.6 ± 15.3) | (5.4 ± 7.6) | (0.07 ± 0.3) | (0) | (0) | |
| Df(1)Hmr−/Df(1)Hmr−; P{Hmr+tp72}16-1/+ | 0.89 | 0.72*** | 0.67*** | 0.56*** | 0.38*** | |
| (N = 61) | (54.0 ± 25.5)*** | (48.6 ± 28.5)*** | (56.7 ± 26.0)*** | (50.4 ± 28.8)** | (67.0 ± 25.5) | |
| 3 | Df(1)Hmr−/Df(1)Hmr− | 0.18 | 0.05 | 0.03 | 0 | 0 |
| (N = 74) | (7.4 ± 10.0) | (0) | (0) | |||
| Df(1)Hmr−/Df(1)Hmr−; P{Hmrtp73}2-2/+ | 0.24 | 0.09 | 0 | 0 | 0 | |
| (N = 74) | (4.6 ± 5.0) | (0) | ||||
| 4 | Df(1)Hmr−/Df(1)Hmr− | 0.29 | 0.06 | 0.01 | 0 | 0 |
| (N = 108) | (1.4 ± 3.5) | (1.0 ± 2.5) | (0) | |||
| Df(1)Hmr−/Df(1)Hmr−; P{Hmrtp73}5-1/+ | 0.32 | 0.11 | 0.04 | 0 | 0 | |
| (N = 105) | (0.6 ± 1.7) | (0.09 ± 0.3) | (0) | |||
| 5 | Df(1)Hmr−/Df(1)Hmr− | 0.61 | 0.40 | 0.24 | 0.11 | 0 |
| (N = 75) | (2.9 ± 5.3) | (0.1 ± 0.4) | (0) | (0) | ||
| Df(1)Hmr−/Df(1)Hmr−; P{Dsim\Hmr+t8.6}2-4/+ | 0.64 | 0.45 | 0.36 | 0.18 | 0.10** | |
| (N = 75) | (17.6 ± 16.4)*** | (5.9 ± 8.8)*** | (1.6 ± 7.3) | (0) | (0) | |
| 6 | Df(1)Hmr−/Df(1)Hmr− | 0.68 | 0.46 | 0.28 | 0.22 | 0.14 |
| (N = 73) | (7.6 ± 14.8) | (0.1 ± 0.5) | (0) | (0) | (0) | |
| Df(1)Hmr−/Df(1)Hmr−; P{Dsim\Hmr+t8.6}4-1/+ | 0.70 | 0.58 | 0.40 | 0.32 | 0.22 | |
| (N = 73) | (16.3 ± 15.5)*** | (3.9 ± 9.9)* | (0) | (0) | (0) | |
| 7 | Df(1)Hmr−/Df(1)Hmr− | 0.71 | 0.46 | 0.33 | 0.22 | 0.14 |
| (N = 70) | (4.1 ± 7.2) | (0.2 ± 0.6) | (0) | (0) | (0) | |
| Df(1)Hmr−/Df(1)Hmr−;P{Dmau\Hmr+t9.4}3-4/+ | 0.80 | 0.63* | 0.54* | 0.46* | 0.32* | |
| (N = 70) | (20.6 ± 16.2)*** | (10.4 ± 16.0)*** | (0.87 ± 3.2) | (0) | (0) | |
| 8 | Df(1)Hmr−/Df(1)Hmr− | 0.59 | 0.40 | 0.26 | 0.10 | 0 |
| (N = 70) | (4.3 ± 8.3) | (0.9 ± 3.2) | (0.3 ± 1.2) | (0) | ||
| Df(1)Hmr−/Df(1)Hmr−; P{Dmau\Hmr+t9.4}1-5/+ | 0.51 | 0.32 | 0.26 | 0.10 | 0.04 | |
| (N = 74) | (16.4 ± 19.9)*** | (4.7 ± 11.1)* | (0.5 ± 1.8) | (0) | (0) | |
For each cross the two genotypes being compared were siblings from a single cross. Single females of the indicated genotypes were mated to two wild-type males. The females were examined for viability and the parents changed to fresh vials on days 5, 10, 15, and 20. Vials were also examined on day 25 and any remaining females discarded. All vials in which the female was alive when examined were kept and total progeny counted. All crosses were done at 27°. See materials and methods for further details.
N can drop during the course of the experiment due to death of the tester males or accidental escape of the flies. N went down slightly (<10%) in all experiments, except for the Df(1)Hmr−, y w v/Df(1)Hmr−, y w v; P{Hmr+tp72}16-1/+ class in cross 2, where N dropped from 61 to 53 during the course of the experiment. The difference in the proportions of live and dead females between the w+ and w classes was tested by a one-tailed Fisher's exact test. *P < 0.05; **P < 0.01; ***P < 0.001, respectively.
For average fertility, the difference between the w+ and w classes was tested by a one-tailed t-test. *P < 0.05; **P < 0.01; ***P < 0.001, respectively. SD, standard deviation.