TABLE 5.

Egg laying defect in Hmr mutant females

Experiment	Genotype of femalesa	Age of females (days old)b	No. of femalesb	Duration of collection (hr)	No. of eggs laid	No. of eggs/ female/hrc	No. of eggs assayed for hatching	No. of eggs hatched	No. eclosedd
1. Df(1)Hmr−	Hmr+	3–4	27	15.75	246	0.58	70	14	12
Repetition 1	Hmr−	3–4	28	15.75	79e	0.18***	73	7	3*
	Hmr+	9–10	23	15.75	105f	0.29	ND
	Hmr−	9–10	19	15.75	18	0.06***	ND
2. Df(1)Hmr−	Hmr+	3–4	40	16	187	0.29	50	10	10
Repetition 2	Hmr−	3–4	35	16	47	0.08***	45	1	1*
	Hmr+	9–10	30	16.5	108	0.22	ND
	Hmr−	9–10	13	16.5	6	0.03***	ND
3. Hmr3	Hmr+	3–4	24	16	134	0.35	134	74	20
Repetition 1	Hmr−	3–4	23	16	41	0.11**	41	10	5
	Hmr+	9–10	20	15	45	0.15	45	11	1
	Hmr−	9–10	11	15	14	0.08	14	1	0
4. Hmr3	Hmr+	3–4	21	15	373	1.18	100	49	25
Repetition 2	Hmr−	3–4	19	15	142	0.50*	100	5	2***
	Hmr+	9–10	19	15	75	0.26	ND
	Hmr−	9–10	14	15	28	0.13	ND

See materials and methods for details of egg collections. Each experiment was performed twice. *P < 0.05; ***P < 0.0001.

^aFor Df(1)Hmr⁻ experiments Hmr⁺ equals Df(1)Hmr⁻/Df(1)Hmr⁻; P{Hmr^+tp72}11-1/+ and Hmr⁻ equals Df(1)Hmr⁻/Df(1)Hmr⁻, from the BC4 stock described in materials and methods. For Hmr³ experiments Hmr⁺ equals w P{EPgy2}Hmr³/w¹¹¹⁸ + and Hmr⁻ equals P{EPgy2}Hmr³/P{EPgy2}Hmr³.

^bAge and number of females at start of egg collection. Experiment 1 was started with 28 females of each genotype and experiment 2 was started with 40 females of each genotype. Experiment 3 was started with 23 (Hmr³/Hmr³) or 24 (Hmr³/+) females. Experiment 4 was started with 19 (Hmr³/Hmr³) or 21 (Hmr³/+) females.

^cWithin each of the two time points of each experiment the number of eggs laid per female was compared between collections from Hmr⁺ and Hmr⁻ females. This ratio was further normalized to the length of collections to facilitate comparisons among collections and experiments. *P < 0.01; **P < 0.001; ***P < 0.0001 using Fisher's exact test (one-tailed).

^dWithin each of the two time points of each experiment the number of eclosed progeny per number of eggs assayed for hatching was compared between collection from Hmr⁺ and Hmr⁻ females using Fisher's exact test (one-tailed).

^ePlus one first-instar larva.

^fPlus 14 first-instar larvae.