TABLE 1.
Bacterial and phage strains, plasmids, and primers
| Names | Relevant genotype and feature | Source |
|---|---|---|
| Bacterial strains | ||
| XL1 Blue | recA1endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZDM15 Tn10 (Tetr)] | Stratagene |
| IN25 | Originally MC4100 | Wang (2006) |
| IN56 | MC4100 (λ cI857), with wt pR′ sequence | Wang (2006) |
| IN158 | MC4100 (λ cI857 Sam7) | Wang (2006) |
| SYP021 | MC4100 (λ cI857 pR′∷cam) | This study |
| SYP026 | MC4100 (λ cI857 pR′-M2) | This study |
| SYP027 | MC4100 (λ cI857 pR′-M1) | This study |
| SYP028 | MC4100 (λ cI857 pR′-M5) | This study |
| SYP029 | IN158 with pZS*1-pR′M1-lacZ | This study |
| SYP030 | IN158 with pZS*1-pR′M2-lacZ | This study |
| SYP032 | IN158 with pZS*1-pR′WT-lacZ | This study |
| SYP033 | IN158 with pZS*1-pR′M5-lacZ | This study |
| SYP037 | IN158 with pZS*1-pR′M3-lacZ | This study |
| SYP041 | IN158 with pZS*1-pR′M4-lacZ | This study |
| SYP043 | MC4100 (λ cI857 pR′-M4) | This study |
| SYP044 | MC4100 (λ cI857 pR′-M3) | This study |
| SYP049 | MC4100(λ cI857 R∷lacZα−) | Shao and Wang (2008) |
| SYP097 | MC4100(λ cI857 pR′-M1 R∷lacZα+) | This study |
| SYP098 | MC4100(λ cI857 pR′-M2 R∷lacZα+) | This study |
| SYP099 | MC4100(λ cI857 pR′-M3 R∷lacZα+) | This study |
| SYP100 | MC4100(λ cI857 pR′-M4 R∷lacZα+) | This study |
| SYP101 | MC4100(λ cI857 pR′-M5 R∷lacZα+) | This study |
| Phages | ||
| All phage strains were thermally induced (see materialsandmethods) from the lysogens above | ||
| Plasmids | ||
| pZS*12-lac | lacZ replacement of luc in pZS*12-luc; the ori gene is derived from low-copy-number pSC101 | This study |
| pS105cmycRφlacZ | With wild-type pR′ | Wang et al. (2003) |
| pZS*1-Cam-lacZ | Cam replacement of pR′ in pZS*1-pR′WT-lacZ | This study |
| pZS*1-pR′WT-lacZ | pZS*12-lacZ with lac operator replaced with wt λ pR′ | This study |
| pZS*1-pR′M1-lacZ | pZS*1-pR′WT-lacZ with wt pR′ replaced with M1 sequence | This study |
| pZS*1-pR′M2-lacZ | pZS*1-pR′WT-lacZ with wt pR′ replaced with M2 sequence | This study |
| pZS*1-pR′M3-lacZ | pZS*1-pR′WT-lacZ with wt pR′ replaced with M3 sequence | This study |
| pZS*1-pR′M4-lacZ | pZS*1-pR′WT-lacZ with wt pR′ replaced with M4 sequence | This study |
| pZS*1-pR′M5-lacZ | pZS*1-pR′WT-lacZ with wt pR′ replaced with M5 sequence | This study |
| pSwtRlacZαblueRz | Insertion of lacZα at the end of phage λ R | Shao and Wang (2008) |
| pSwtRlacZαwhiteRz | LacZα− mutant of pSwtRlacZαblueRz | Shao and Wang (2008) |
| Primers | ||
| See supplemental materials for sequences | ||