TABLE 2.
Late promoter activity, lysis time, burst size, assembly rate, and growth rate of phage λ pR′ mutants
| Late promoter activitya
|
Burst size (phages/cell) | Assembly rateb (phages/min) | Growth rate (hr−1)
|
||||
|---|---|---|---|---|---|---|---|
| Name | Uninduced | Induced | Lysis time (min) | Competing | Standard | ||
| wt | 6.9 ± 0.6 (B)c | 247.0 ± 17.8 (100%)d (A)c | 52.8 ± 1.1 (D)c | 153.9 ± 7.2 (A, B)c | 6.21 | 2.72 ± 0.090 (A)c | 2.68 ± 0.097 (A)c |
| M1 | 9.6 ± 0.5 (A) | 208.4 ± 6.5 (84%) (B) | 52.5 ± 1.0 (D) | 157.0 ± 11.5 (A, B) | 6.41 | 2.69 ± 0.093 (A) | 2.67 ± 0.072 (A) |
| M2 | 10.2 ± 0.5 (A) | 216.0 ± 4.8 (87%) (B) | 52.8 ± 1.7 (D) | 151.0 ± 27.1 (B) | 6.09 | 2.67 ± 0.142 (A) | 2.66 ± 0.083 (A) |
| M3 | 4.5 ± 0.3 (C) | 149.4 ± 2.2 (60%) (C) | 56.5 ± 1.0 (C) | 163.1 ± 19.7 (A, B) | 5.72 | 2.77 ± 0.062 (A) | 2.68 ± 0.091 (A) |
| M4 | 3.0 ± 0.5 (D) | 75.9 ± 3.6 (31%) (D) | 61.5 ± 1.0 (B) | 186.1 ± 6.8 (A) | 5.56 | 2.60 ± 0.071 (A) | 2.75 ± 0.060 (A) |
| M5 | 3.4 ± 0.2 (C, D) | 14.6 ± 0.4 (6%) (E) | 103.3 ± 2.8 (A) | 104.2 ± 1.7 (C) | 1.38 | 1.28 ± 0.209 (B) | 2.73 ± 0.119 (A) |
All values, if possible, are shown with mean and 95% confidence intervals, which are calculated with standard errors corrected for small sample sizes (see materials and methods).
β-Galactosidase activity in Miller units.
The assembly rate is estimated using b/(tL − e), where b is the burst size, tL is the lysis time, and e is the eclipse period, which is set to 28 min (Wang 2006).
Different letters in the same column show means that are significantly different from each other, using the Tukey–Kramer HSD test at the α = 0.05 level, as implemented in JMP (v. 5.0.1a for MacOS).
Percentages inside the parentheses denote the promoter activities relative to that of the wt.