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. 2009 Apr;181(4):1477–1491. doi: 10.1534/genetics.108.099663

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Copyright © 2009 by the Genetics Society of America

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Figure 3.— — Expression, translation, and silencer localization of S. cerevisiae SIR1 and the four paralogs of SIR1 in S. bayanus. (A) Clustal W alignment of N terminus of Sir1 orthologs as provided from the Saccharomyces Genome Database. (B) Immunoblot of S. cerevisiae Sir1 immunoprecipitated from yeast expressing C-terminally HA-tagged SIR1 or mutated sir1 from a plasmid. (C) Immunoblot of FLAG-tagged Sir1 and Kos proteins immunoprecipitated. Kos1 migrated anomalously slower than Sir1. Kos1 had a calculated pI of 7.25 compared to 5.55 of Sir1, 5.2 of Kos2, and 7.9 of Kos3. (D) Chromatin immunoprecipitations of FLAG-tagged S. bayanus Sir1 paralogs at HML and HMR silencer.