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PCR detection of the ccdO157 system in the E. coli species. The ccdO157 system is located between the folA and the apaH gene (not on scale). Its presence was detected by PCR using the P40 and P93 primers that are complementary to the ORFs that are flanking the ccdO157 system. Another set of primers (P43 and P47) complementary to the ccdAO157 and ccdBO157 ORFs was used to detect it at other potential chromosomal locations in strains that did not carry ccdO157 between folA and apaH.
