TABLE 3.
The variants of CcdAO157 and CcdBO157 proteins
| Strain serogroup | No. of isolates | Antitoxin activity | Amino acid sequence |
|---|---|---|---|
| CcdA variants | |||
| 157 (EDL933 reference strain) | — | + | MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF------------------------------------------------------------------------------------------------------ 77 |
| 2/4/5/6/11/15/16/17/22/23/24/25/33/45/51/52/55/58/81/83/85/86/89/112/114/115/120/134/139/142/145/150/153/157/161/166/176 | 37 | + | MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF------------------------------------------------------------------------------------------------------- 77 |
| 44/133 | 2 | + | MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF------------------------------------------------------------------------------------------------------- 77 |
| 1/7/75/79 | 4 | + | MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF------------------------------------------------------------------------------------------------------- 77 |
| 171 | 1 | + | MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSEEYRSF-------------------------------------------------------------------------------------------------------77 |
| 119 | 1 | + | MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAAIRWREENAEAIAALNQLADETGCFSDEYRSF------------------------------------------------------------------------------------------------------- 77 |
| 102 | 1 | + | MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF------------------------------------------------------------------------------------------------------- 77 |
| 138 | 1 | − | MTAKRTTQSVTVTVDRELVNRARDAGLNMSAPLRLRSMLNLKNMQQHVGVKRTQKLSLR-------------------59 |
| Strain serogroup | No. of isolates | Toxic activity | Amino acid sequence |
| CcdB variants | |||
| 157 (EDL933 reference strain) | — | + | MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 44/55/157/171 | 4 | + | MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 24/33/52/112/114/145/176/115 | 8 | + | MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 11/58/161 | 3 | + | MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 133 | 1 | + | MQFTVYHSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 85/89 | 2 | + | MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 2/45/83 | 3 | − | MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI------------------------------------------------------- 40 |
| 81 | 1 | + | MQFTVYHSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLLKGALDFMLDGI----------------------------------------------------------------- 104 |
| 1/4/5/6/16/22/25/75/79/120/134/142/150 | 13 | + | MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 102 | 1 | NT | MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKF----------------------------------------------------------------------------------------------------- 83 |
| 138/153 | 2 | − | MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI------------------------------------------------------------------ 104 |
| 166 | 1 | − | MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALGTKF----------------------------------------------------------------------------------------------------- 83 |
| 17/15 | 2 | − | MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGS------------------------- 62 |
| 23/51/86/119/139 | 5 | − | MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGS------------------------ 62 |
| 7 | 1 | NT | MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIE------------------------------------------------------ 41 |
NT, not tested; +, active for either toxicity or antitoxicity; −, not active for either toxicity or antitoxicity. Amino acid variation as compared to amino acid sequence of the reference CcdAO157 and CcdBO157 proteins is indicated in italics. The CcdA and CcdB variants that have been tested experimentally are represented in boldface type.