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. 2009 Apr 3;284(14):9027–9038. doi: 10.1074/jbc.M805685200

FIGURE 2.

FIGURE 2.

PS1 mutations stably expressed in neuronal cell lines determine BACE1 up-regulation. SH-SY5Y, stably transfected with pcDNA3.1, PS1 wt, and PS1 mutant S170F, were analyzed for BACE1 mRNA (A), BACE1 protein levels (B), and BACE1 activity (C). Similarly, M17 neuronal cells, stably expressing PS1 A246E mutant, PS1 wt, and pcDNA3.1, were analyzed: D, BACE1 mRNA; E, BACE1 protein levels; F, BACE1 activity. G and H, sandwich enzyme-linked immunosorbent assay for Aβ40 and -42, performed analyzing conditioned medium of SH-SY5Y stable cells (G) and M17 stable cells (F). The Aβ42/40 ratio is also presented. Data represent the mean and S.E. of three or more experiments, performed on two different stable cell clones; significance is relative to empty vector control (CTR); for enzyme-linked immunosorbent assay, p values are specified in the figure. Statistical analysis was performed with ANOVA and Bonferroni post-test.