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. 2009 Apr 3;284(14):9093–9098. doi: 10.1074/jbc.M900019200

FIGURE 5.

FIGURE 5.

TBP and TFIIB are both required to induce slipping on a bubble template. A, leaving either TBP or TFIIB out of transcription reactions eliminates transcript slippage. Reactions were performed on the wild-type template in the presence of GTP (productive 12-nt product is indicated with an asterisk). The factors were omitted from reactions as indicated. A darker exposure of the same gel is shown to the right. B, TBP and TFIIB function together to cause slipping. Reactions were performed on the wild-type template in the presence of GTP, with the factors added in the combinations indicated. C, higher concentrations of either TBP or TFIIB do not force transcript slippage. TBP, TFIIB, and both factors together were added to reactions at 1×, 3×, and 10× the standard concentrations. Transcription occurred on the wild-type template in the absence of GTP.