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A TATA box, but not a BRE, is required for TBP/TFIIB-induced transcript slippage. A, schematics of the mutant templates that lacked either the BRE or the BRE and TATA box. B, complexes assembled on the ΔBREΔTATA template do not undergo transcript slippage. Transcription in the absence and presence of TBP and TFIIB was monitored from the mutant and wild-type templates in the absence of GTP. The expected 10-nt product is indicated by an asterisk.
