PRMT1 is required for PXR transcriptional activity. A, PXR
activity in PRMT1(-/-) ES cells. Gal4-driven luciferase reporter gene and
Gal4-mPXR were transiently transfected into mouse PRMT1-null ES cells or
wild-type ES cells. The transfected cells were treated with the receptor
agonist PCN (10 μm, 24 h). Luciferase activity was determined by
a luminometer. *, statistically significant difference (t
test, p < 0.01). The data are the means ± S.D. of three
independent results. B and C, the effect of siRNA knockdown
of PRMT1 on PXR transcriptional activity. PXR-HepG2 cells were transfected
with CYP3A4-luciferase. Two siRNAs targeting different sequences of
PRMT1 (756–773 and 353–371) were used to knockdown PRMT1.
Scrambled siRNA was used as the control (B and upper panel
of C). The total PRMT1 protein expression was analyzed by Western
blotting with PRMT1 antibody. Western blot with α-tubulin antibody was
shown for loading control (C, lower panel). Lane 1,
siPRMT1–11; lane 2, siPRMT1–28; lane 3, control.
The reporter gene expression was measured by luciferase assay. *,
statistically significant difference (t test, p < 0.01).
The data are the means ± S.D. of three independent results.