FIGURE 6.

AKT is required for NS4B-mediated SREBP activation. A, total cell lysates harvested from both vector stable and NS4B-Myc stable cells were immunoblotted with the indicated antibodies (upper panel). Triplicate experimental data of p-AKT levels were quantified (lower panel). B, Huh7 cells were transiently transfected with either vector or NS4B-Myc expression plasmid. At 24 h after transfection, cells were harvested and immunoblotted with the indicated antibodies (upper panel). Data from triplicate experiments were quantified and each bar represents the average intensity of p-AKT level (lower panel). C, both vector stable and NS4B-Myc stable cells were transfected with either FAS-Luc (upper panel) or SRE-Luc (lower panel) reporter plasmid. At 24 h after transfection, cells were either left untreated (Me₂SO) or treated with LY294002 (5 μm) for an additional 12 h. Cells were harvested and then luciferase activities were determined. Data represent the mean of two independent experiments. D, total cellular extracts used in C were immunoblotted with the indicated antibodies. Quantification of the band intensity was determined by using a calibrated GS-800 densitometer.