TABLE 5.
Number of HBs formed at substituted sites of MCP-02 and E495 in the dynamic structures
|
Residues, MCP-02, E495
|
280 K
|
310 K
|
||||
|---|---|---|---|---|---|---|
| MCP-02 | E495 | Δ | MCP-02 | E495 | Δ | |
| N2D | 7 | 6 | –1 | 10 | 5 | –5 |
| L16I | 5 | 6 | 1 | 5 | 6 | 1 |
| D25N | 7 | 9 | 2 | 4 | 16 | 12 |
| S29T | 5 | 3 | –2 | 5 | 13 | 8 |
| T32N | 6 | 9 | 3 | 10 | 13 | 3 |
| N37T | 11 | 11 | 0 | 17 | 13 | –4 |
| A38T | 2 | 4 | 2 | 4 | 5 | 1 |
| R41K | 11 | 9 | –2 | 17 | 10 | –7 |
| F79Y | 4 | 8 | 4 | 5 | 9 | 4 |
| L92I | 4 | 3 | –1 | 3 | 4 | 1 |
| A95P | 2 | 3 | 1 | 5 | 3 | –2 |
| I155V | 1 | 7 | 6 | 3 | 7 | 4 |
| N157S | 2 | 6 | 4 | 5 | 10 | 5 |
| P160S | 2 | 11 | 9 | 2 | 15 | 13 |
| S182T | 3 | 2 | –1 | 5 | 4 | –1 |
| N216S | 8 | 9 | 1 | 11 | 10 | –1 |
| Y218S | 5 | 4 | –1 | 5 | 5 | 0 |
| Y230F | 5 | 3 | –2 | 6 | 3 | –3 |
| P241S | 0 | 0 | 0 | 7 | 2 | –5 |
| Q246E | 6 | 6 | 0 | 4 | 6 | 2 |
| I250K | 4 | 5 | 1 | 4 | 6 | 2 |
| S261T | 3 | 9 | 6 | 2 | 7 | 5 |
| G263S | 1 | 4 | 3 | 1 | 5 | 4 |
| V264T | 4 | 12 | 8 | 3 | 13 | 10 |
| G265N | 2 | 9 | 7 | 2 | 11 | 9 |
| N285S | 10 | 3 | –7 | 12 | 13 | 1 |
| D286A | 7 | 4 | –3 | 7 | 6 | –1 |
| A292S | 3 | 5 | 2 | 4 | 8 | 4 |
| D305S | 5 | 8 | 3 | 18 | 21 | 3 |
| A307G | 5 | 5 | 0 | 18 | 12 | –6 |
| Sum of substituteda | 140 | 183 | 43 | 204 | 261 | 57 |
| Sum of Proteinb | 778 | 887 | 109 | 1023 | 1117 | 94 |
| Ratioc | 18.0% | 20.6% | 39.4% | 19.9% | 23.4% | 60.6% |
a
The sum of HBs of the 30 substituted sites
b
The sum of HBs of all 307 sites in the protein
c
The percentage of HBs of the substituted sites in all the HBs in protein