TABLE 2.
Number of free thiols in WT and C87S GrxS12 under various redox conditions The untreated column is indicative of the protein thiol content measured after purification. GrxS12 was reduced by DTTred and subsequently oxidized by DTTox or GSSG. After each treatment (DTTred, DTTox, and GSSG), samples were desalted. The thiol content per protein was quantified by 5,5′-dithiobis(nitrobenzoic acid). Data are represented as mean ± S.D. (n = 4). ND, not determined.
| Untreated | DTTred treatment | DTTox treatment | GSSG treatment | |
|---|---|---|---|---|
| GrxS12 | 0.821 ± 0.078 | 1.98 ± 0.269 | 1.9 ± 0.1 | 0.808 ± 0.029 |
| GrxS12 C87S | 0.115 ± 0.017 | 1.056 ± 0.125 | ND | 0.132 ± 0.053 |