TABLE 1.
Summary of kinetic constants determined in this study
Kinetic constants are the average of three independent trials, except for some of the experiments with SAGA Gcn5-Y413A, which were the average of two trials. For experiments with two trials, the results of each trial is reported in parentheses instead of reporting a standard deviation. Half-saturation concentration was calculated from K1/n. The apparent turnover rate constant, kcat,app, was determined from Vmax/[E]0. Data marked with an asterisk is from previously published work (17).
| Octamer | DNA | Enzyme | [Half-saturation] | kcat,app | Cooperativity coefficient |
|---|---|---|---|---|---|
| nm | min−1 | ||||
| Wild-type* | 208-12 Arrays | SAGA WT Gcn5 | 33.6 ± 5.0 | 1.42 ± 0.15 | 1.97 ± 0.15 |
| H4-Lys-16Ac | 208-12 Arrays | SAGA WT Gcn5 | 19.7 ± 1.7 | 2.64 ± 0.29 | 1.31 ± 0.09 |
| Wild-type | 208-12 Arrays | SAGA Gcn5-Y413A | 104.7 ± 29.7 | 1.46 ± 0.15 | 1.01 ± 0.22 |
| H4-Lys-16Ac | 208-12 Arrays | SAGA Gcn5-Y413A | 98.1 (80.3, 115.9) | 1.50 (1.39, 1.61) | 1.14 (1.13, 1.14) |
| Wild-type | 208-12 Arrays | Ada2/Ada3/WT Gcn5 | 74.0 ± 14.1 | 1.00 ± 0.07 | 1.63 ± 0.17 |
| Wild-type | 208-12 Arrays | Ada2/Ada3/Gcn5-Y413A | 201.1 ± 17.4 | 1.00 ± 0.06 | 0.91 ± 0.15 |
| Tetra-Ala H3/WT H3* | 196-1 Monos | SAGA WT Gcn5 | 38.9 ± 6.9 | 1.07 ± 0.07 | 0.95 ± 0.03 |
| Tetra-Ac H3/WT H3* | 196-1 Monos | SAGA WT Gcn5 | 11.5 ± 2.4 | 1.12 ± 0.08 | 0.99 ± 0.10 |
| Tetra-Ala H3/WT H3 | 196-1 Monos | SAGA Gcn5-Y413A | 32.8 (32.6, 32.9) | 0.96 (0.94, 0.97) | 0.91 (0.84, 0.98) |
| Tetra-Ac H3/WT H3 | 196-1 Monos | SAGA Gcn5-Y413A | 30.6 (26.4, 34.7) | 0.94 (0.89, 0.98) | 0.96 (0.89, 1.02) |