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. 2000 Jan 4;97(1):349–353. doi: 10.1073/pnas.97.1.349

Figure 1.

Figure 1

Schematic for GLGI. (A) In this process, first-strand cDNA synthesized by oligo(dT) is used for PCR. In the first cycle, the template with the SAGE tag binding site is annealed by the sense primer and extended to the end of the template. In the second cycle, extension occurs only from the anchored oligo(dT) primer annealed and paired correctly at the beginning of poly(dA) sequences. Exponential amplification occurs only for the template with the SAGE tag binding site. (B) The result of GLGI will be the conversion of 10 bases of SAGE tag to a hundred bases of 3′ cDNA fragment.