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. 2009 Apr 3;284(14):9529–9539. doi: 10.1074/jbc.M809393200

FIGURE 9.

FIGURE 9.

Effect of Chordin-like 1 and SB203580 on expression of p21Cip1/WAF1, p53, phospho-Rb, and Apo J. A, Western blot analysis of the protein levels of p21Cip1/WAF1, p53, and phospho-Rb (pRb) in oxidant-treated ARPE-19 cells, with or without Chordin-like 1 or SB203580. The higher levels of p21Cip1/WAF1 and p53 protein induced by oxidants were reduced by Chordin-like 1 or SB203580 treatment. In each experiment, equal loading was demonstrated by immunoblot for GAPDH. B, analysis of the expression of senescence marker gene, Apo J, in oxidant treated ARPE-19 cells with or without inhibitors using real time PCR. The expression of Apo J was about 6-fold higher in tBH-treated cells and about 4-fold higher in H2O2-treated cells than the expression in nonstressed control ARPE-19 cells. The increased expression of Apo J in stressed ARPE-19 cells was markedly suppressed when Chordin-like 1 or SB203580 was added into the medium. **, p < 0.01.