FIGURE 4.

Effects of KLF5 knockdown on cyclin D1 expression, cell proliferation and cell cycle progression. A, expression levels of KLF5 and other cellular factors by RNA interference. Expression levels of KLF5, cyclin D1, and cyclin A are shown as bar graphs after densitometric analysis. B, the actual number of cells was counted in triplicate, and the average was shown as line plots (^**, p < 0.01). C, staining with 7-amino-actinomysin D followed by analysis with flow cytometer and cell cycle distribution. The error bar denotes S.D.