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. 2009 Apr 3;284(14):9549–9557. doi: 10.1074/jbc.M806230200

FIGURE 5.

FIGURE 5.

Effects of KLF5 on SMC apoptosis. A, uncleaved and cleaved levels of apoptotic caspase-3 analyzed by Western blot. Note that cleaved caspase-3 was specifically not seen in the KLF5 group under serum deprivation. Amounts of loaded protein were confirmed with β-actin blotting. Adv, adenoviral. B, the effects of KLF5 on apoptosis under anisomycin treatment (2 μmol/liter). The right lower insets show the same distribution of cells in each group counterstained with propidium iodide. The right bar graph shows TUNEL-positive apoptotic cell rate (**, p < 0.01). The error bar denotes S.D. C and D, the effects of KLF5 overexpression on VSMC apoptosis in the later (14 days) (C) or in the early (48 h) (D) phase in rat carotid arteries after balloon injury and adenoviral transfection as assessed by TUNEL staining. Brown-stained nuclei were TUNEL-positive and were slightly counterstained with methyl green. Intestine samples were stained as positive control. The TUNEL-positive rate was calculated as the ratio of TUNEL-positive nuclei/methyl green-positive nuclei (scale bar, 20 mm, **, p < 0.01). The error bar denotes S.D.