Table 4.

The primers used for the bisulfite sequencing of bCSH1.

Region	Strand	Sequence	Annealing
Region 1	Forward	5'-AGGGAAGATTTTTTGGAGAAGG-3'	60
	Reverse	5'-AATAATAACCTTCAAATAACCAATACAC-3'
Region 2	Forward	5'-AAGAGTTTGTATGGATTTTTTTAGA-3'	60
	Reverse	5'-CCACACTCTTCCTCAATAATAATAA-3'
Region 3	Forward	5'-TTTGATAATTGTTTATTGAATGATTTATTA-3	60
	Reverse	5'-CAAATTCACTCCTAACCTATCTTTTCT-3'
Region 4	Forward	5'-AGTTTGTTAATAAATGAATTTTTTTT-3'	56
	Reverse	5'-CTTACTTTTTCCTCTTTTCTACCCTAAA-3'
Region 5	Forward	5'-GTTTGGGGTTGAATATTTATTATT-3'	60
	Reverse	5'-TACCTACTTCTATTTAATACCAATT-3'
Region 6	Forward	5'-ATGTTGTTTATTATAGGGTGTATA-3'	56
	Reverse	5'-TTATATCTTTTACAATTTTAATACTAA-3'