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. 2009 Mar 5;10:19. doi: 10.1186/1471-2199-10-19

Table 5.

The primers used for the bisulfite sequencing of bPRP1.

Region Strand Sequence Annealing
Region 1 Forward 5'-GGGATTGTTGTTGTTGTTGTTAAGT-3' 60
Reverse 5'-AAAACTATCTCTTTCTCCATACTAATACCT-3'
Region 2 Forward 5'-TTGTGTATGAGATAGTAAAAGAGATATTGA-3' 60
Reverse 5'-CAATAAAAAACCAAAAAAACTATAATTACA-3'