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Spectral changes associated with the enzymic conversion of cob(II)alamin to AdoCbl by LrPduOF112H. Arrows and increasing darkness in the spectra represent successive time points after reaction was initiated by the addition of ATP (light gray, 1min; black, 11 min). LrPduOF112H facilitates the reduction of cob(II)alamin (as evidenced by the decrease in absorbance at 473 nm) however there is a lag in the generation of AdoCbl (as evidenced in initial lag in absorbance at 525 nm).
