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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Mol Cell Cardiol. 2009 Jan 21;46(5):621–635. doi: 10.1016/j.yjmcc.2009.01.005

Figure 1. Wnt3a induces spindle-shaped morphology change and upregulates SM22α mRNA accumulation in C3H10T1/2 multipotent mesenchymal cells.

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Panel A, C3H10T1/2 cells were treated either with vehicle, 15 ng/ml recombinant purified Wnt3a, 5 ng/ml recombinant purified TGF-β1, or with both ligands as indicated for 7 days as indicated. Panel B, within 4 days of treatment, 15 ng/ml Wnt3a significantly upregulates SM22α mRNA accumulation. Panel C, western blot analysis confirms that Wnt3a increases SM22α protein levels as well (see also Figs. 1E and 2B). Duplicate blots are shown, representative of the 4 independent blots analyzed by digital image analysis. Unlike Wnt3a, Wnt1 and Wnt5a do not upregulate SM22α mRNA accumulation (panel D) or SM22 protein levels (panel E). All Wnt treatments were at 15 ng/ml.