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. Author manuscript; available in PMC: 2010 May 1.

Published in final edited form as: J Mol Cell Cardiol. 2009 Jan 21;46(5):621–635. doi: 10.1016/j.yjmcc.2009.01.005

Panel A, C3H10T1/2 cells were transfected either with control siRNA or a validated β-catenin siRNA as indicated, and then treated with either vehicle or 15 ng/ml Wnt3a for 24 hours. Total cellular RNA was extracted, and mRNA accumulation of SM22α and PPARγ quantified by RT-qPCR with normalization to 18S rRNA. Data are presented as the mean +/− error from five independent transfections and treatments was analyzed for each group. Note that siRNA to β-catenin inhibited Wnt3a induction of SM22α. **, p < 0.01 by post-hoc Tukey’s test from all other conditions. Panel B, western blot analysis confirmed that β-catenin siRNA impaired β-catenin protein induction in transfected C3H10T1/2 cells. Duplicate blots are shown, representative of the 4 independent blots analyzed by digital image analysis (separate gels indicated by the vertical black line). Data are derived from several independent gels run in parallel. Panel C, siRNA directed toward all Smad2 isoforms also inhibited induction of SM22α gene expression by Wnt3a.

Panel A, C3H10T1/2 cells were transfected either with control siRNA or a validated β-catenin siRNA as indicated, and then treated with either vehicle or 15 ng/ml Wnt3a for 24 hours. Total cellular RNA was extracted, and mRNA accumulation of SM22α and PPARγ quantified by RT-qPCR with normalization to 18S rRNA. Data are presented as the mean +/− error from five independent transfections and treatments was analyzed for each group. Note that siRNA to β-catenin inhibited Wnt3a induction of SM22α. **, p < 0.01 by post-hoc Tukey’s test from all other conditions. Panel B, western blot analysis confirmed that β-catenin siRNA impaired β-catenin protein induction in transfected C3H10T1/2 cells. Duplicate blots are shown, representative of the 4 independent blots analyzed by digital image analysis (separate gels indicated by the vertical black line). Data are derived from several independent gels run in parallel. Panel C, siRNA directed toward all Smad2 isoforms also inhibited induction of SM22α gene expression by Wnt3a.