Figure 3.

Identification of transposon insertions that alleviate cell toxicity associated with CB1 production that do not map on dnaJ. A: Fluorescence histograms of MC4100A, GS102 (MC4100A dinG::Tn5), and GS103 ((MC4100A nhaR::Tn5) cells expressing CB1-GFP at room temperature for approximately 5 h. Histograms correspond to a total population of 10,000 cells. M: arithmetic mean; a.u: arbitrary units. B: Cell density of MC4100A, GS102 and GS103 cultures expressing CB1-GFP at room temperature for approximately 5 h. The reported values correspond to the average of four replica experiments and the error bars represent one standard deviation from the mean value. OD₆₀₀: optical density at 600 nm. C: Western blots on total cell lysates demonstrating the production levels of DnaJ in MC4100A, GS101, GS102, and GS103 cells. Cells were grown to mid-log phase at 37°C and DnaJ production levels were probed with an anti-DnaJ antibody. An equal number of cells were loaded on each lane as judged by OD₆₀₀ measurements. [Color figure can be seen in the online version of this article, available at www.interscience.wiley.com.]