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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):5098–5106. doi: 10.4049/jimmunol.0803446

Figure 1. AMCase protects epithelial cells from apoptosis.

Figure 1

a. Cultured A549 cells were kept for serum starvation in RPMI1640 medium without FCS for 48h at 37°C or were kept in RPMI1640 medium with addition of FasL (100 ng/mL). Where indicated AMCase-transfection was performed (AMCase +). Where indicated, AMCase bioactivity in the cell-culture supernatant was inhibited by allosamidin (10μM). Apoptosis was assessed by annexin V (left panel) or FACS TUNEL (right panel) staining. * p<0.05, Student’s t-test.

b. Representative FACS dot-plots of Annexin V vs PI staining are shown. Where indicated AMCase-transfection was performed (AMCase +). Where indicated, A549 cells underwent serum starvation.

c. Annexin V (left panel) or FACS TUNEL (right panel) staining was performed with non-transfected A549 cells treated with medium or AMCase (10μg/ml). Where indicated, AMCase bioactivity in the cell-culture supernatant was inhibited by allosamidin (10μM). Representative FACS dot-plots of FACS TUNEL staining are shown. * p<0.05, Student’s t-test.