Levels of phosphorylated Akt (a), Annexin V expression (b) and DNA fragmentation (c) were analyzed in A549 cells treated with increasing doses of AMCase (100ng/ml, 1μg/ml, 10μg/ml and 100μg/ml). Where indicated, the cells underwent serum starvation (left panel) or were treated with FasL (100 ng/ml) (right panel) as described above in detail. Where indicated, PI3K was inhibited using wortmannin (1μM). Data from three independent experiments is shown. * p<0.05, Mann-Whitney U test