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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):5098–5106. doi: 10.4049/jimmunol.0803446

Figure 6. Enzymatic activity is not required for anti-apoptotic and chemokine-eliciting effects of AMCase.

Figure 6

Enzymatic activity was measured in cell culture supernatants as described in detail in the methods section (a). Annexin V (b) and intracellular phosphorylated Akt (c) were analyzed by flow cytometry in A549 cells transfected with the empty vector, with full-length human AMCase (AMCase+ Chitinolytic+) or with mutated, enzymatically inactive AMCase (AMCase+ Chitinolytic-). CCL2/MCP-1 levels were quantitated in supernatants of A549 cells A549 cells transfected with the empty vector, with full-length human AMCase. *p<0.05, Student’s t-test. Where indicated, AMCase bioactivity in the cell-culture supernatant was inhibited by allosamidin (10μM). Data from three independent experiments is shown.