Figure 3.

Multilineage engraftment of hoxb4-expanded human cord blood CD34⁺ cells. Representative flow cytometry profile of engrafted BM cells in mice received BSA or T-hoxb4-H treated human cord blood CD34⁺ cells. Human cord blood CD34+ cells were cultured for 4 days with 50nM BSA or T-hoxb4-H. Cells were then collected and injected into sublethally irradiated NOD/SCID mice, with each recipient received cells equivalent to 10⁴ or 5×10³ starting CD34⁺ cells in a SRC assay as detailed in Material and Methods. Recipient BM cells were harvested eight weeks later and stained for human myeloid (CD14/CD15) and lymphoid (CD19) markers.