(Previously published, Journal of Cerebral Blood Flow and Metabolism 26:654, 2006.) Effects of stroke on cytokines secreted from stimulated splenocytes and blood cells. Spleens and blood were collected 6 h and 22 h after vascular occlusion and immune cells were stimulated for 48 h with plate-bound anti-CD3/CD28 antibodies. Supernatants were evaluated for levels of secreted factors, including TNF-α, IFN-γ, IL-6, MCP-1, IL-2, and IL-10. * Indicates a significant difference in expression in stroke mice versus sham-treated mice.