(Previously published, Journal of Immunology 176:6523, 2006.) Increase in CD4+FoxP3± Treg cells in spleen 96 h after MCAO. Splenocytes were obtained from naïve, sham MCAO, and MCAO mice 96 h after stroke and evaluated for staining with antibodies to CD4 and FoxP3, a marker for Treg cells. Note increase in CD4+FoxP3+ T cells in MCAO mice (upper right quadrant).