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. Author manuscript; available in PMC: 2010 Mar 18.
Published in final edited form as: FEBS Lett. 2009 Feb 24;583(6):971–977. doi: 10.1016/j.febslet.2009.02.028

Fig. 1.

Fig. 1

CDK11p46 phosphorylates eIF3f at more than one site during apoptosis. (A) Kinase assay: CDK11p46 was immunoprecipitated from apoptotic A375 cells and incubated with GST-eIF3f or eIF3fS46A. (B) Kinase assay was performed with GST or four truncated eIF3f proteins as substrates. (C) Diagram of Mov34 domain and four deletion constructs of eIF3f. (D) MS identification of Thr119 phosphorylation. (E) Kinase assays were performed with eIF3f-1 (T119) and eIF3f-1 T119A (T119A) as substrates. (F) Staurosporine treated A375 cells were immunoprecipitated with eIF3 antibody followed by immunoblot with phosphothreonine or eIF3f antibody.