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Schematic of Langmuir monolayer imprinting. The unordered surfactant layer at the air/water interface (A) interacts with the protein, inducing demixing, allowing specific binding pockets to form (B). The layer is transferred to a hydrophobic support, which hinders further lateral movement of the monolayer components hence holding the defined pockets in place (C). Specific rebinding of protein is demonstrated (D), upon removal of the template.
