Table 1.
Antibody | Antibody type | Immunizing antigen | Immunoreactivity in Western immunoblotting | Immunoreactivity in immunohistochemistry |
---|---|---|---|---|
Anti-DMP1-N-9B6.3a | Monoclonal | 37 kDa (N-terminal) | Yes | Very weak |
Anti-DMP1-N-859b | Polyclonal | Oligopeptide (residues 101–121) | Yes | Yes |
Anti-DMP1-C-785c | Polyclonal | Oligopeptide (residues 471–485) | Yes | Yes |
Anti-DMP1-C-8G10.3d | Monoclonal | 57 kDa (C-terminal) | No | Yes |
This monoclonal antibody was used to detect the NH2-terminal fragment of DMP1 by Western immunoblotting analysis. The characterization of this antibody has been described previously [13]
This polyclonal antibody was used to detect the NH2-terminal fragment of DMP1 by immunofluorescence (primary utilization) and Western immunoblotting (secondary utilization) in this study. This antibody was recently generated in our laboratory (see “Materials and Methods”)
This polyclonal antibody was used to detect the COOH-terminal fragment of DMP1 by Western immunoblotting analysis in this study. A detailed description of the characteristics of this antibody was reported previously [19]
This monoclonal antibody was used to detect the COOH-terminal fragment of DMP1 by immunofluorescence analysis. The characterization of this antibody has been reported [20]