Skip to main content
. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: Calcif Tissue Int. 2008 May 17;82(5):401–410. doi: 10.1007/s00223-008-9140-7

Table 1.

Antibodies used in this study

Antibody Antibody type Immunizing antigen Immunoreactivity in Western immunoblotting Immunoreactivity in immunohistochemistry
Anti-DMP1-N-9B6.3a Monoclonal 37 kDa (N-terminal) Yes Very weak
Anti-DMP1-N-859b Polyclonal Oligopeptide (residues 101–121) Yes Yes
Anti-DMP1-C-785c Polyclonal Oligopeptide (residues 471–485) Yes Yes
Anti-DMP1-C-8G10.3d Monoclonal 57 kDa (C-terminal) No Yes
a

This monoclonal antibody was used to detect the NH2-terminal fragment of DMP1 by Western immunoblotting analysis. The characterization of this antibody has been described previously [13]

b

This polyclonal antibody was used to detect the NH2-terminal fragment of DMP1 by immunofluorescence (primary utilization) and Western immunoblotting (secondary utilization) in this study. This antibody was recently generated in our laboratory (see “Materials and Methods”)

c

This polyclonal antibody was used to detect the COOH-terminal fragment of DMP1 by Western immunoblotting analysis in this study. A detailed description of the characteristics of this antibody was reported previously [19]

d

This monoclonal antibody was used to detect the COOH-terminal fragment of DMP1 by immunofluorescence analysis. The characterization of this antibody has been reported [20]