Fig. 2.

D234A substitution did not block DMP1 processing. For these experiments, proteins (DMP1 and its processed fragments) purified by ion exchange chromatography were used. Note that DMP1-PG was eluted in different chromatographic fractions and thus was not included in these samples. a About 6 μg of purified protein was loaded onto each lane for Stains-All staining. Note that the pattern of protein bands is similar between the D234A substitution (lane 1) and the normal control group (lane 2). b About 3 μg of purified protein was loaded onto each lane for Western immunoblotting using the antibody against the COOH-terminal region of mouse DMP1 (anti-DMP1-C-785). Lane 1: D234A substitution; lane 2: normal control group. Note the presence of the DMP1 COOH-terminal fragment (approximately 57 kDa) in both groups.