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Western immunoblotting of dentin extracts using monoclonal anti-BSP IgG1 10D9.2 as a probe. Bone sialoprotein (BSP) was detected in G1 (A), E (B), and G2 (C) extracts of dentin. BSP was least abundant in G1 extract and most abundant in G2 extract. The positive control (Cont) was 2 μg of BSP purified from rat bone.
