Figure 1.

Specificity and time course of in vivo CTL priming after application of peptide to tape-stripped skin. (A) Cervical lymph node cells (effectors) obtained from mice (n = 5) immunized 10 days earlier with TRP-2, MUT1, or OVA (96 μg per mouse) either through intact earlobes or earlobes tape-stripped 12 hr earlier were subjected to CTL assay using L^Kb or L^tk- target cells pulsed with TRP-2, MUT1, or OVA. CTL assays were performed at effector-to-target ratio of 10. (B) TRP-2, MUT1, or OVA (96 μg per mouse) was painted on intact earlobes (0 hr) and those tape-stripped 12, 24, and 48 hr before. Cervical lymph node cells (effectors) obtained 10 days after peptide application were tested in a CTL assay using L^Kb target cells pulsed with the peptide used for immunization. Data are expressed as the average of the mean of two independent experiments. E/T, effector-to-target ratio.