Figure 2.
Augmentation of specific CTL induction by peptide application topically on tape-stripped skin. (A) Indicated doses of TRP-2 peptide dissolved in acetone/oil were painted on tape-stripped earlobes (1st) and abdominal skin (2nd) 2 weeks later. Cervical lymph node cells and splenocytes were assayed for lysis against TRP-2-pulsed LKb target cells at an effector-to-target ratio of 10. (B) Mice were immunized with TRP-2 (96 μg per mouse) dissolved in PBS, acetone/oil, or 70% ethanol by painting on earlobes or with intradermal injection into the base of earlobes, which were either untreated or tape-stripped. Cervical lymph node cells were assayed with TRP-2-pulsed LKb target cells at an effector-to-target ratio of 20. All data are expressed as mean ± SE of two independent experiments.