Figure 3.

Protection against tumor cell challenge after peptide immunization via barrier-disrupted skin. (A) Mice (n = 10) were treated with TRP-2 or MUT1 on untreated or tape-stripped skin. B16 or 3LL tumor cells (1 × 10⁵ cells per mouse) were inoculated s.c. into TRP-2- or MUT1-treated mice, respectively, and tumor sizes were measured every 3 days. (B) CD4⁺ or CD8⁺ cell-depleted, or control rat IgG-treated mice (n = 10) were painted with TRP-2 or MUT1 on tape-stripped skin. B16 or 3LL tumor cells (1 × 10⁵ cells per mouse) were inoculated s.c. into TRP-2- or MUT1-treated mice, respectively, and tumor sizes were measured every 3 days.