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MUT1 and TRP-2 peptide-specific pCTL frequencies. Splenocytes obtained from untreated mice or mice treated twice with MUT1 or TRP-2 at tape-stripped skin were subjected to limiting dilution analysis. The number of wells containing peptide-specific CTLs determined by CTL assay was counted. OVA-pulsed LKb target cells were used as control.
