Table 3.

Specific activity values (μg glucose reducing sugar equivalent/(μg CBH II enzyme × min)) for parent and selected thermostable CBH II enzyme chimeras

CBH II Enzyme	μg of reducing sugar/(μg of enzyme) × min) × 102
Humicola insolens (Parent 1)	4.0 ± 0.5
Hypocrea jecorina (Parent 2)	13 ± 2
Chaetomium thermophilum (Parent 3)	5.0 ± 0.5
HJPlus (Chimera 12222332)	10.0 ± 0.8
Chimera 11113132	4.5 ± 0.5
Chimera 13311332	6.7 ± 0.3

Error bars show standard errors, where standard error is defined as standard dev/sqrt (n), for between 5 and 8 replicates per CBH II enzyme. Two-hour reaction, 3 mg enzyme/g PASC, 50°C, 25 mM sodium acetate, pH 4.8.