Fig. 2.

DARPP-32 gene silencing efficiency of GNR-siRNA^D nanoplex in DAN cells. Quantitative real-time PCR (Q-PCR) data showing a >80% suppression of DARPP-32 gene expression in DAN cells that were transfected with the GNR-siRNA^D nanoplex. Relative expression of mRNA species was calculated using the comparative CT method. Data are the mean ± SD of 3 separate experiments done in duplicate. Statistical significance was determined using ANOVA based on comparison between the siPORT-siRNA^D (positive control), GNR-siRNA^D nanoplex, and the siPORT with scrambled siRNA (negative control). A significant decrease in DARPP-32 gene expression was observed up to 1 week posttransfection, with maximum suppression observed at 96 h posttransfection.