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. 2009 Mar 23;106(14):5546–5550. doi: 10.1073/pnas.0901715106

Fig. 5.

Fig. 5.

Immunofluorescent studies on neuronal cells. Immunofluorescent staining for DARPP-32 (A–D) and ERK-1 (E and F) in transfected and nontransfected DAN cells. (A) DARPP-32 expression in nontransfected DAN cells. (B) DARPP-32 expression in GNR-siRNAD nanoplex transfected DAN cells. (C) Morphine-treated (10−7 M) but nontransfected DAN cells. (D) Morphine-treated (10−7 M) DAN cells, transfected with GNR-siRNAD. (E) Morphine-treated (10−7 M) untransfected DAN cells showing ERK-1 expression. (F) Morphine-treated (10−7 M) GNR-siRNAD tranfected DAN cells showing ERK-1 expression. Our results show (A) morphine treatment enhances the DARPP-32 levels, and (B) GNR-mediated transfection of siRNA against DARPP-32 reduces not only the levels of DARPP-32 expression but also the expression of its downstream effector molecule ERK-1 in DAN cells.