Table 1.

SPR affinity measurements for TCR 1G4 and Fabs 3M4E5, 3M4F4, and Fab T1

Analyte	A2-NY-ESO-1 peptide	Ka, mol-1 s-1	Kd, s-1	KD, nM
1G4 TCR	9C	87,500	0.357	4,080
	9V	53,425	0.078	1,460
3M4E5	9C	93,700	0.004225	47
	9V	80,850	0.00362	46
3M4F4	9C	68,800	0.00651	95
	9V	73,700	0.00467	63
Fab T1	9C	138,375	0.000674	2
	9V	249,000	0.000835	4

NY-ESO-1_157–165 peptides carried a cysteine (9C) or valine(9V) at position 9.